Validation of a UV Spectrometric Method for the Assay of Tolfenamic Acid in Organic Solvents

Table 3

Analytical parameters for the determination of TA in various organic solvents.

Solvent	Linearity range, conc. (mg%)	Corr. coeff.	Slope	SE of slope	Intercept	SE of intercept	SD of intercept	Recovery range (%)	Accuracy (%)  ± SD	Precision (%RSD)	LOD; (mg%)	LOQ; (mg%)
Acetonitrile	0.2–0.9 (0.52–2.36)	0.99985	9.08 × 10^3	0.00419	−0.00102	0.00385	0.01089	98.73–101.07	99.90 ± 0.7899	0.7907	3.96 (0.10)	1.20 (0.31)
Methanol	0.2–0.8 (0.52–2.09)	0.99994	1.04 × 10^4	0.00273	0.03714	0.00277	0.00734	99.24–100.87	99.99 ± 0.6113	0.6113	2.34 (0.06)	7.08 (0.19)
Ethanol	0.2–0.8 (0.52–2.09)	0.99992	9.43 × 10^3	0.00281	0.00218	0.00286	0.00757	99.06–100.85	99.96 ± 0.5969	0.5971	2.65 (0.07)	8.03 (0.21)
Acetone	0.3–1.0 (0.79–2.62)	0.99956	8.78 × 10^3	0.00690	−0.06210	0.00764	0.02021	98.15–101.40	100.11 ± 1.2665	1.2651	7.60 (0.20)	2.30 (0.60)
1-Propanol	0.2–0.8 (0.52–2.09)	0.99971	1.08 × 10^4	0.00613	0.02508	0.00624	0.01651	98.93–101.18	99.92 ± 0.8370	0.8377	5.02 (0.13)	1.52 (0.40)
1-Butanol	0.2–0.8 (0.52–2.09)	0.99953	1.00 × 10^4	0.00730	0.00595	0.00743	0.01966	95.92–102.39	99.76 ± 2.0754	2.0805	6.47 (0.17)	1.96 (0.51)
1-Hexanol	0.3–1.0 (0.79–2.62)	0.99984	7.93 × 10^3	0.00378	−0.01175	0.00418	0.01107	98.78–100.99	100.05 ± 0.7348	0.7344	4.61 (0.12)	1.40 (0.37)
Benzyl alcohol	0.2–0.8 (0.52–2.09)	0.99983	9.39 × 10^3	0.00413	0.02471	0.00421	0.01113	99.15–101.27	99.95 ± 0.7502	0.7506	3.91 (0.10)	1.19 (0.31)
1-Octanol	0.2–0.8 (0.52–2.09)	0.99996	9.12 × 10^3	0.00203	0.01139	0.00206	0.00546	99.11–100.71	100.01 ± 0.5566	0.5565	1.97 (0.05)	5.98 (0.16)
Dichloromethane	0.2–0.8 (0.52–2.09)	0.99992	1.10 × 10^4	0.00328	−0.05499	0.00334	0.00884	99.03–101.31	100.06 ± 0.7268	0.7264	2.66 (0.07)	8.07 (0.21)
Ethyl acetate	0.2–0.8 (0.52–2.09)	0.99987	8.99 × 10^3	0.00338	−0.00287	0.00344	0.00911	98.88–101.69	99.96 ± 0.8841	0.8845	3.34 (0.09)	1.01 (0.26)
Chloroform	0.2–0.8 (0.52–2.09)	0.99976	8.93 × 10^3	0.00465	0.02979	0.00473	0.01252	98.88–101.30	99.95 ± 0.8556	0.8560	4.63 (0.12)	1.40 (0.37)
Toluene	0.2–0.9 (0.52–2.36)	0.99990	8.54 × 10^3	0.00312	−0.00004	0.00287	0.00811	98.67–101.32	100.10 ± 0.8768	0.8760	3.13 (0.08)	9.50 (0.25)

.
SE = standard error.
SD = standard deviation.
Recovery (%) = (amount found/amount added) × 100
(amount found = absorbance − intercept/slope).
Accuracy (%) = mean recovery range.
% of relative standard deviation (%RSD) = (SD/mean) × 100.
Limit of detection (LOD) = 3.3 × (SD of intercept/slope).
Limit of quantification (LOQ) = 10 × (SD of intercept/slope).