Research Article
Validation of a UV Spectrometric Method for the Assay of Tolfenamic Acid in Organic Solvents
Table 3
Analytical parameters for the determination of TA in various organic solvents.
| Solvent | Linearity range, conc. (mg%) | Corr. coeff. | Slope | SE of slope | Intercept | SE of intercept | SD of intercept | Recovery range (%) | Accuracy (%) ± SD | Precision (%RSD) | LOD (mg%) | LOQ (mg%) |
| Acetonitrile | 0.2–0.9 (0.52–2.36) | 0.99985 | 9.08 × 103 | 0.00419 | −0.00102 | 0.00385 | 0.01089 | 98.73–101.07 | 99.90 ± 0.7899 | 0.7907 | 3.96 (0.10) | 1.20 (0.31) | Methanol | 0.2–0.8 (0.52–2.09) | 0.99994 | 1.04 × 104 | 0.00273 | 0.03714 | 0.00277 | 0.00734 | 99.24–100.87 | 99.99 ± 0.6113 | 0.6113 | 2.34 (0.06) | 7.08 (0.19) | Ethanol | 0.2–0.8 (0.52–2.09) | 0.99992 | 9.43 × 103 | 0.00281 | 0.00218 | 0.00286 | 0.00757 | 99.06–100.85 | 99.96 ± 0.5969 | 0.5971 | 2.65 (0.07) | 8.03 (0.21) | Acetone | 0.3–1.0 (0.79–2.62) | 0.99956 | 8.78 × 103 | 0.00690 | −0.06210 | 0.00764 | 0.02021 | 98.15–101.40 | 100.11 ± 1.2665 | 1.2651 | 7.60 (0.20) | 2.30 (0.60) | 1-Propanol | 0.2–0.8 (0.52–2.09) | 0.99971 | 1.08 × 104 | 0.00613 | 0.02508 | 0.00624 | 0.01651 | 98.93–101.18 | 99.92 ± 0.8370 | 0.8377 | 5.02 (0.13) | 1.52 (0.40) | 1-Butanol | 0.2–0.8 (0.52–2.09) | 0.99953 | 1.00 × 104 | 0.00730 | 0.00595 | 0.00743 | 0.01966 | 95.92–102.39 | 99.76 ± 2.0754 | 2.0805 | 6.47 (0.17) | 1.96 (0.51) | 1-Hexanol | 0.3–1.0 (0.79–2.62) | 0.99984 | 7.93 × 103 | 0.00378 | −0.01175 | 0.00418 | 0.01107 | 98.78–100.99 | 100.05 ± 0.7348 | 0.7344 | 4.61 (0.12) | 1.40 (0.37) | Benzyl alcohol | 0.2–0.8 (0.52–2.09) | 0.99983 | 9.39 × 103 | 0.00413 | 0.02471 | 0.00421 | 0.01113 | 99.15–101.27 | 99.95 ± 0.7502 | 0.7506 | 3.91 (0.10) | 1.19 (0.31) | 1-Octanol | 0.2–0.8 (0.52–2.09) | 0.99996 | 9.12 × 103 | 0.00203 | 0.01139 | 0.00206 | 0.00546 | 99.11–100.71 | 100.01 ± 0.5566 | 0.5565 | 1.97 (0.05) | 5.98 (0.16) | Dichloromethane | 0.2–0.8 (0.52–2.09) | 0.99992 | 1.10 × 104 | 0.00328 | −0.05499 | 0.00334 | 0.00884 | 99.03–101.31 | 100.06 ± 0.7268 | 0.7264 | 2.66 (0.07) | 8.07 (0.21) | Ethyl acetate | 0.2–0.8 (0.52–2.09) | 0.99987 | 8.99 × 103 | 0.00338 | −0.00287 | 0.00344 | 0.00911 | 98.88–101.69 | 99.96 ± 0.8841 | 0.8845 | 3.34 (0.09) | 1.01 (0.26) | Chloroform | 0.2–0.8 (0.52–2.09) | 0.99976 | 8.93 × 103 | 0.00465 | 0.02979 | 0.00473 | 0.01252 | 98.88–101.30 | 99.95 ± 0.8556 | 0.8560 | 4.63 (0.12) | 1.40 (0.37) | Toluene | 0.2–0.9 (0.52–2.36) | 0.99990 | 8.54 × 103 | 0.00312 | −0.00004 | 0.00287 | 0.00811 | 98.67–101.32 | 100.10 ± 0.8768 | 0.8760 | 3.13 (0.08) | 9.50 (0.25) |
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. SE = standard error. SD = standard deviation. Recovery (%) = (amount found/amount added) × 100 (amount found = absorbance − intercept/slope). Accuracy (%) = mean recovery range. % of relative standard deviation (%RSD) = (SD/mean) × 100. Limit of detection (LOD) = 3.3 × (SD of intercept/slope). Limit of quantification (LOQ) = 10 × (SD of intercept/slope).
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