Research Article
Evaluation of Actin-1 Expression in Wild Caught Wuchereria bancrofti-Infected Mosquito Vectors
Table 2
Cycle threshold (Ct) values and calculations for relative fold change in the expression of the actin-1 gene.
| Samples | Ct test | Mean Ct (test) | Ct ref (GAPDH) | Mean Ct (ref) | ∆Ct test (mean Ct of test‐ mean Ct ref) | ∆∆Ct (∆Ct test‐∆Ct control) | 2−∆∆Ct | SD | SEM |
| Infected A | 15.87 | | 18.93 | | | | | | | 15.80 | 15.81 | 18.86 | 18.87 | −3.06 | | | | | 15.77 | | 18.82 | | | | | | | | | | | | −2.28 | 4.85 | 0.0573 | 0.033 |
| Control A | 16.33 | | 17.12 | | | | | | | 16.28 | 16.25 | 17.08 | 17.04 | −0.79 | | | | | 16.13 | | 16.91 | | | | | | |
| Infected B | 15.6 | | 18.47 | | | | | | | 15.68 | 15.67 | 18.55 | 18.60 | −2.87 | | | | | 15.74 | | 18.6 | | | | | | | | | | | | −2.03 | 4.09 | 0.0132 | 0.008 |
| Control B | 15.78 | | 16.62 | | | | | | | 15.85 | 15.82 | 16.68 | 16.65 | −0.84 | | | | | 15.82 | | 16.66 | | | | | | |
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A: Anopheles gambiae and B: Culex quinquefasciatus.
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