No Polymorphism in Plasmodium falciparum K13 Propeller Gene in Clinical Isolates from Kolkata, India

Table 1

Primers and PCR conditions of primary and nested PCR of K13 gene.

Primer name	Primer sequence (5′-3′)	Mg^2+	PCR programme
		Conc.	Denaturation		Annealing		Elongation		Number of cycles
		(mM)	Temp.	Time	Temp.	Time	Temp.	Time	1st	2nd
			(°C)	(min)	(°C)	(min)	(°C)	(min)	PCR	PCR
K13_PCR_F; K13_PCR_R	CGGAGTGACCAAATCTGGGA; GGGAATCTGGTGGTAACAGC	3	94	0:30	60	1:30	72	1:30	40	—
K13_N1_F; K13_N1_R	GCCAAGCTGCCATTCATTTG; GCCTTGTTGAAAGAAGCAGA	2.5	94	0:30	60	1:30	72	1:30	—	40