Sesamol: A Natural Phenolic Compound with Promising Anticandidal Potential

Figure 5

Phenotypic susceptibility assays to reveal the effect of Ses on the calcineurin dependent phenotypes in C. albicans. (a) Spot assay with and without Ses (0.552 mg/mL) as controls. (b) Spot assay with Ses at elevated pH 10 showing hypersensitivity to C. albicans. (c) Spot assay depicting the loss of growth in cells treated with serum (50% w/v) along with Ses. (d) Spot assay showing loss of growth under ER stress by DTT (20 mM) in presence of Ses. (e) Spot assays showing sensitivity in the presence of Ses under various ionic stress conditions with LiCl (0.4 M), CaCl₂ (0.3 M), and NaCl (1 M). (f) Spot assay demonstrating the attenuated growth of C. albicans in the presence of Ses with membrane perturbing agent SDS (0.02% w/v). (g) Spot assay demonstrating no growth loss at elevated temperature at 37°C and 42°C in the presence of Ses.

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