Chronic Osteoporotic Pain in Mice: Cutaneous and Deep Musculoskeletal Pain Are Partially Independent of Bone Resorption and Differentially Sensitive to Pharmacological Interventions

Figure 3

Efficacy of chronic bisphosphonate (pamidronate) treatment on body weight and bone mineral density. Mice were treated with pamidronate (0.25 mg/kg) or a saline vehicle 5 days/week for 5 weeks, beginning 6 months after undergoing ovariectomies or sham surgeries. Pamidronate has no effect on body weight after either 1 or 5 weeks of treatment (a) but results in a significant reversal of ovariectomy-induced bone mineral density decreases after 5 weeks of treatment that is not observed after 1 week of treatment (b). Data are expressed as means ± standard error of the mean. OVX: ovariectomized; BisP: bisphosphonate (pamidronate); ; and OVX with bisphosphonate pamidronate versus saline vehicle control, two-way repeated measures analysis of variance (factors = group time) followed by Tukey’s test for multiple comparisons.

(a) Body weight

(b) Bone mineral density