miR-26a-5p Attenuates Oxidative Stress and Inflammation in Diabetic Retinopathy through the USP14/NF-κB Signaling Pathway

Figure 5

miR-26a-5p overexpression alleviates retinal injury of mice with DR by regulating the USP14/NF-κB signaling. (a) H&E staining was performed to observe the pathological changes of murine retinas and to quantify retinal thickness in the normal, DR, DR + NC-agomir, or DR + miR-26a-5p agomir groups. Red arrows: the number of retinal ganglion cells. Yellow arrow: vessel-like structure; angiogenesis in the DR group. (b–e) ELISA was conducted to measure (b) VEGF level and (c–e) the accumulation of proinflammatory cytokine (TNF-α, IL-1β, and IL-6) in murine retinas of the normal, DR, DR + NC-agomir, or DR + miR-26a-5p agomir groups. (f–h) Measurement of SOD, CAT, and MDA levels in murine retinas of the normal, DR, DR + NC-agomir, or DR + miR-26a-5p agomir groups was performed using specific commercial kits. (i) Western blotting was performed to quantify the protein levels of USP14, p-IκBα, and IκBα in murine retinas of the normal, DR, DR + NC-agomir, or DR + miR-26a-5p agomir groups. n = 10/group. , , and .

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