Application of ImageJ in Optical Coherence Tomography Angiography (OCT-A): A Literature Review

Figure 4

To determine the vessels and delineate their boundaries in preparation for analysis, ImageJ is utilized. In the choriocapillaris (CC) layer, an OCT-A image identified a choroidal neovascularization (CNV) complex (A). It was binarized utilizing the contrast thresholding technique (B). Subsequently, the outer portion of the CNV was eliminated by selecting its border (C). By integrating images (B) and (C), the CNV region was rendered (D) amenable to fractal dimension and vessel density analysis. The image must be in skeletonized form in order to derive additional parameters such as vessel length density. In conclusion, the skeletonized form was constructed using the binary image (E).