Application of ImageJ in Optical Coherence Tomography Angiography (OCT-A): A Literature Review

Figure 2

The application of ImageJ to elucidate the structure of choroidal neovascularization (CNV). Optical coherence tomography angiography (OCT-A) image of a CNV network in the outer retinal layer (A). (B) The preceding CNV in the layer of choriocapillaris (CC). (C) Use of a rolling ball background to increase the brightness of the CC image background. (D) The CNV was separated using the image calculator's subtraction mode and the combination of images (A) and (C). (E) Subsequently, employ the image calculator to combine mode images (A) and (D) in order to enhance the visibility of the CNV. (F) A bandpass filter was applied to image (D) to improve the structural view. (G) Subsequently, the image was prepared for thresholding (F) as the background became darker in the presence of a rolling ball subtraction method. Ultimately, the implementation of the ISODATA thresholding procedure elucidated the structure of the CNV.