Modulation of Hypoxia-Inducible Factors and Vascular Endothelial Growth Factor Expressions by Superfood Camu-Camu (Myrciaria dubia) Treatment in ARPE-19 and Fetal Human RPE Cells

Figure 5

Comparison of camu-camu and vitamin C effects on suppression of HIF-1α protein expression. HIF-1α activity induced by 200 μM CoCl₂ and suppression by doxorubicin. Treatment with 1 μg/mL camu-camu comprises 5.85 ×10⁻² μg/mL of vitamin C. (a) Camu-camu suppresses HIF-1α protein expression in ARPE-19 cells. (b) Quantification of the blots shows that the administration of camu-camu suppressed increased HIF-1α protein expression under CoCl₂ in ARPE-19 cells (n = 3, biological and technical). (c) HIF-1α expression under CoCl₂ in fhRPE cells. (d) Quantification of the blots in fhRPE (n = 3, biological and technical). ^# compared with CoCl₂ + MQ. and compared with no CoCl₂ treatment. The bar graphs present means with ± standard deviation. The data were analyzed using two-tailed Student’s t-test for comparison. MQ: Milli-Q® pure water; Topo: topotecan; DXR: doxorubicin; Vit C: vitamin C.

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