Modulation of Hypoxia-Inducible Factors and Vascular Endothelial Growth Factor Expressions by Superfood Camu-Camu (Myrciaria dubia) Treatment in ARPE-19 and Fetal Human RPE Cells

Figure 4

Comparison of hypoxia-responsive gene expression by camu-camu and vitamin C. Quantitative analyses (n = 3–5 per group, biological) using ARPE-19 cells reveal significant changes in HIF-1α, HIF-2α, and BNIP3 mRNA expression after 7 hours of 10 μg/mL and 30 μg/mL camu-camu treatment. Vitamin C was added at 1.755 μg/mL. Low-dose camu-camu treatment comprised 10 μg/mL of camu-camu, and high-dose camu-camu treatment (30 μg/mL) contained 1.755 μg/mL of vitamin C. HIF-1α, HIF-2α, and BNIP3 mRNA-suppressive effects of camu-camu treatment were generally greater than those of vitamin C treatment. ^#, ^##, and ^### compared with no treatment. , , and compared with camu-camu treatments and vitamin C treatment. NS: no significant. The bar graphs present means with ± standard deviation. The data were analyzed using two-tailed Student’s t-test for comparison.

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