The Effect of A2E on the Ca²⁺-PKC Signaling Pathway in Human RPE Cells Exposed to Blue Light

Figure 1

(a) Primary human RPE cells showing high levels of confluence and pigmentation after 24 (h). (b) Third-generation RPE cells are spindle-shaped, triangular, or irregular in shape, with a reduced number of cytoplasmic pigment granules. (c) Third-generation RPE cells fixed in 4% paraformaldehyde and incubated with an anti-RPE65 antibody. DAPI (blue) was also added to stain the nuclei.

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