The Concentrations of Interleukin-6, Insulin, and Glucagon in the Context of Obesity and Type 2 Diabetes and Single Nucleotide Polymorphisms in IL6 and INS Genes
Table 2
The conditions for PCR and restriction enzyme digestion.
The initial denaturation-95°C for 5 min, denaturation-95°C for 40 s, annealing-67.4°C for 35 s, elongation-72°C for 25 s, the final elongation72°C for 10 min
rs1800795
Forward primer-5′ TGA CTT CAG CTT TAC TCT TTG T 3′ Reverse primer-5′ CTG ATT GGA AAC CTT ATT AG 3′
The initial denaturation-95°C for 15 min, denaturation-95°C for 40 s, annealing-55°C for 35 s, elongation-72°C for 40 s, the final elongation-72°C for 10 min