FAM64A Promotes Osteosarcoma Cell Growth and Metastasis and Is Mediated by miR-493

Figure 5

FAM64A is the target of miR-493 in OS. (a) MiR493 binding sequence in 3′UTR of the FAM64A gene and the mutant sequence. (b) The pattern of FAM64A 3-UTR WT/MT inserted to psiCHECK2. (c) 293T cells were cotransfected with psiCHECK2-FAM64A 3′-UTR WT or psiCHECK2-FAM64A 3′-UTR MT and miR493 mimics or NC. The luciferase activity was measured. (d) and (e) mRNA levels of FAM64A in MG-63 and U-2 OS cells. (f) Detection of miR-493 expressions in OS tissues and nontumor tissues using RT-RCR; (f) correlation analysis of miR-493 levels with FAM64A mRNA levels. . UTR: untranslated region; WT: wild type; MT: mutant; NC: negative control.

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