Urotensin-II-Targeted Liposomes as a New Drug Delivery System towards Prostate and Colon Cancer Cells

Figure 6

Cell proliferation of all developed formulations in prostate (DU145, PC3, and LNCaP) and colon (WIDR and LoVo) cancer cell lines. Cancer cells were seeded in serum-containing media in 96- well plates at the density of 2 × 10³ cells/well. After 24 h incubation at 37°C, cells were treated with 10 μM of free doxo. Lipo: plain liposomes; LipoUT: PEGylated liposomes conjugated with UT-II peptide; Lipo-doxo: PEGylated liposomes-encapsulated doxo; LipoUT-doxo: PEGylated liposomes conjugated with UT-II peptide and encapsulated with doxo. Lipo, Lipo-doxo, LipoUT, and LipoUT-doxo for 72 h. Cell viability was assessed by MTT assay, as described in Materials and Methods. .