Mutated WT1, FLT3-ITD, and NUP98-NSD1 Fusion in Various Combinations Define a Poor Prognostic Group in Pediatric Acute Myeloid Leukemia

Table 3

Multivariate analysis.

Cox regression analysis - Event-free survival
Parameters	Hazard ratio	95% confidence interval		p value
		Lower limit	Upper limit
WT1 mutation	0.79	0.41	1.53	0.479
FLT3-ITD AR ≥ 0.4	1.55	0.69	3.51	0.288
WT1 mutation, FLT3-ITD ≥ 0.4 and	3.88	1.42	10.66	0.008
NUP98-NSD1 interaction
t(8;21) and/or inv(16)	0.51	0.27	0.96	0.037
Unsatisfactory early response to treatment	1.31	0.79	2.18	0.294
HSCT	0.25	0.1	0.64	0.004
Cox regression analysis - Overall survival
WT1 mutation	0.84	0.35	2.06	0.710
FLT3-ITD ≥ 0.4	1.51	0.57	4.02	0.404
WT1 mutation, FLT3-ITD ≥ 0.4 and	3.42	1.04	11.21	0.042
NUP98-NSD1 interaction
t(8;21) and/or inv(16)	0.45	0.16	1.31	0.143
Unsatisfactory early response to treatment	1.21	0.61	2.42	0.589
HSCT	1.18	0.51	2.73	0.700

WT1, Wilms tumor 1; FLT3-ITD, fms related tyrosine kinase 3-internal tandem duplication; NUP98-NSD1, Nucleoporin-Nuclear Receptor Binding SET Domain Protein 1 fusion gen; t, translocation; inv, inversion; HSCT, hematopoietic stem cell transplantation.
Unsatisfactory early response to treatment was defined as persistence of >5% blasts in bone marrow at day 15 and/or 28 after treatment. hematopoietic stem cell transplantation events at first complete remission or after no-response to other treatments were included in the multivariate analysis as a time-dependent variable.