Downregulation of PGC-1α Prevents the Beneficial Effect of EET-Heme Oxygenase-1 on Mitochondrial Integrity and Associated Metabolic Function in Obese Mice
Table 1
(a) EET-agonist treatment (4 weeks) WT and Ln PGC-1α(sh) mice. Body weights, fasting blood glucose, blood pressure, oxygen consumption, and respiratory quotient of mice fed regular chow or HF diet for 8 weeks. EET-A administered for the last 4 weeks. (b) EET-agonist treatment (8 weeks) WT mice. Body weights, blood glucose, oxygen consumption, and respiratory quotient of mice fed regular chow or HF diet for 24 weeks. EET-A administered for the last 8 weeks.
(a)
Variable
Lean
HF
HF+EET-A
HF+EET-A+Ln PGC-1α(sh)
BW (g)
25.8 ± 0.35
30.2 ± 0.85
23.9 ± 0.19
29.0 ± 1.47
FBS (mg/dl)
107.5 ± 4.84
209.5 ± 9.84
156.0 ± 11.06
263.5 ± 21.71
BP (mm Hg)
104.0 ± 1.34
148.2 ± 3.01
1.05 ± 1.11
137.0 ± 2.33
VO2 (ml/kg/min)
53.92 ± 2.82
45.54 ± 1.82
77.17 ± 3.38
46.46 ± 3.72
RQ (CO2/O2)
0.74 ± 0.003
0.77 ± 0.007
0.74 ± 0.01
0.78 ± 0.003
Values are means ± SEM of 6 mice per group. Differences at were considered significant. versus lean, versus HF, and versus HF+EET-A. Body weight (BW). Blood pressure (BP). Fasting blood sugar (FBS). Oxygen consumption (VO2). Respiratory quotient (RQ).
(b)
Variable
Lean
HF
HF+EET-A
BW (g)
28.68 ± 1.21
56.63 ± 0.88
32.43 ± 0.83
FBS (mg/dl)
109.5 ± 4.84
286 ± 8.11
100.2 ± 11.32
BP (mm Hg)
105.8 ± 2.87
156.7 ± 6.87
113.5 ± 4.44
VO2 (ml/kg/min)
56.2 ± 2.65
33.77 ± 1.19
57.95 ± 3.12
RQ (CO2/O2)
0.73 ± 0.006
0.8 ± 0.011
0.75 ± 0.007
Values are means ± SEM of 3–5 mice per group. Differences at were considered significant. versus lean; versus HF. Body weight (BW). Fasting blood sugar (FBS). Blood pressure (BP). Oxygen consumption (VO2). Respiratory quotient (RQ).
