Downregulation of PGC-1α Prevents the Beneficial Effect of EET-Heme Oxygenase-1 on Mitochondrial Integrity and Associated Metabolic Function in Obese Mice

Figure 4

Effect of short-term EET-A treatment on insulin receptor phosphorylation and AKT and AMPK signaling in adipose tissue of mice fed HF diet. (a) Representative western blots and ((b) to (f)) densitometry analyses of WBs in adipose tissue of mice; (b) insulin receptor phosphorylation IRp-Tyr-972; (c) insulin receptor phosphorylation IRp-Tyr-1146; (d) phosphorylated protein kinase B (pAKT); (e) phosphorylated adenosine monophosphate protein kinase (pAMPK) proteins in mice fed a normal diet lean and mice fed a high fat (HF) diet treated with EET-A. Results are mean ± SE, , versus lean, and versus HF.

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