Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization

Table 2

Analysis of variance (ANOVA) for the custom design of response surface methodology to synthesize magnetic particles maximizing the amount of relative protein loading and magnetically attracted mass.

Source	DF	Sum of squares adjusted	Mean square adjusted	value	value
Model	9	1715.75	190.639	89.45	0.001
Lineal	3	451.64	150.546	70.64	0.001
Chitosan	1	332.10	332.096	155.83	0.001
Sonication	1	34.66	34.659	16.26	0.002
Cross-linker	1	72.28	72.285	33.92	0.001
Square	3	89.31	29.769	13.97	0.001
Chitosan × chitosan	1	88.53	88.529	41.54	0.001
Sonication × sonication	1	3.45	3.452	1.62	0.227
Cross-linker × cross-linker	1	0.09	0.088	0.04	0.842
Interaction	3	984.29	328.097	153.95	0.001
Chitosan × sonication	1	722.13	722.131	338.84	0.001
Chitosan × cross-linker	1	39.02	39.025	18.31	0.001
Sonication × cross-linker	1	48.61	48.608	22.81	0.001
Error	12	25.57	2.131
Total	21	1741.33