Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization
Table 1
Custom design of response surface methodology to synthesize magnetic particles maximizing the amount of relative protein loading and magnetically attracted mass.
Treatment
Chitosan (% )
Sonication time (min)
Genipin (% )
Relative protein absorbed by support (% )
Relative mass attracted (% )
PLMA (%)
1
1.0
3.0
0.100
10.40 ± 0.34
94.43 ± 0.11
73.26 ± 0.00
2
0.5
6.5
0.055
69.59 ± 0.42
98.18 ± 0.18
90.28 ± 0.03
3
1.0
10.0
0.010
50.11 ± 1.64
96.11 ± 0.25
84.05 ± 0.22
4
1.0
6.5
0.055
15.50 ± 0.23
96.84 ± 0.09
74.51 ± 3.28
5
0.5
6.5
0.010
100.00 ± 5.59
99.07 ± 0.38
98.25 ± 1.11
6
1.5
3.0
0.055
71.58 ± 0.47
98.27 ± 0.01
90.82 ± 0.13
7
1.5
6.5
0.055
1.55 ± 0.24
95.66 ± 2.72
72.05 ± 2.10
8
0.5
3.0
0.010
0.63 ± 0.18
100.00 ± 0.12
75.08 ± 0.05
9
1.5
3.0
0.010
60.82 ± 0.21
96.67 ± 0.01
87.04 ± 0.06
10
1.0
10.0
0.100
0.69 ± 0.05
96.46 ± 0.05
72.45 ± 0.05
11
1
6.5
0.055
15.50 ± 0.08
96.24 ± 0.22
74.52 ± 3.01
PLMA: [(PL + MA) × 0.5] ± standard deviation () of relative protein loading and relative mass attracted.
