Electrospun Collagen: A Tissue Engineering Scaffold with Unique Functional Properties in a Wide Variety of Applications
Figure 7
Analysis of Type I collagen α chain content. (a) Representative SDS gel depicting the affect of various degrees of heat denaturation on soluble collagen. Lane 1 = MW standards, 2 = control sample maintained and stored at 4°C, 3 = sample heated to 50°C for 1 hr, 4 = 60°C, 5 = 70°C, 6 = 80°C, and 7 = 90°C. (b) Densitometric analysis of α chain content from denaturation experiments. (c) Densitometric analysis (c) ratio of α 1(III) to α 1(I), (d) ratio of α 1(III) to α 2(I), (e) ratio of α 1(I) to α 2(I) as a function of thermal insult. Dotted lines in (c)–(e) depicts the actual ratio of the α chains present in collagen recovered immediately after electrospinning (no thermal insult).
