Effects of 5-Hydroxymethylcytosine Epigenetic Modification on the Stability and Molecular Recognition of VEGF i-Motif and G-Quadruplex Structures

Table 5

Thermodynamic properties of VEGF G4 modification sequences in the presence and absence of 100 mM KCl and 40% ACN alone or combined. Marked increases in thermal stability were noted upon addition of 100 mM KCl alone and with 40% ACN as compared to their own controls. Thermal stability was not substantially different between 5-hmC modifications and wild-type. No pronounced changes in transition state dynamics were observed. Error bars are omitted for clarity and are <10% of the values shown; ND = not determinable.

	Δ (°C)	(kcal/mol)	(°C)	(kcal/mol)	(°C)	(kcal/mol)	(°C)	(kcal/mol)
Control	62	21	50	12	ND	ND	73	16
100 mM KCl	84	22	82	27	76	30	84	15
100 mM KCl + 40% ACN	>100	25	>100	19	>100	20	95	12