Research Article
In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
Table 1
SELEX scheme for selection of a bromacil-specific MRE.
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In vitro selection process for obtaining a bromacil-specific MRE. Immobilization target (IT) is bromacil bound to magnet beads. Immobilization substrate (IS) is streptavidin-coated magnetic beads plus blocked biotin reagent. Competitive elution (CE) is removal of bound ssDNA from target-coated magnetic beads by free pesticide in solution. INT-1 or NT-1 is the 1st immobilized or free negative target 6-amino-3-ethyl methyl uracil. INT-2 or NT-2 is the 2nd immobilized or free negative target 1-methyl uracil. Times listed are incubation times in hours (hrs), minutes (min), or seconds (sec). |