Multipyrene Tandem Probes for Point Mutations Detection in DNA

Table 2

Thermal stability^a of duplexes between tandem probes and their components with fcDNA target^b.

Tandem	IVb + IIb	IVb + IIa	IVa + IIb	IVa + IIa	IVb + II	IV + IIb	IVa + II	IV + IIa	IV + II	II
𝑁^cpyr	4	3	3	2	2	2	1	1	0	—
𝑇𝑚, °C	51.2	50.8	49.3	49.7	45.8	49.7	53.4	48.8	53.3	42.0

aConditions of thermal stability determination are listed in experimental section. ^bFor fcDNA sequence see Figure 1. ^cNumber of pyrene residues at the junction.