Population-Sequencing as a Biomarker of Burkholderia mallei and Burkholderia pseudomallei Evolution through Microbial Forensic Analysis

Figure 3

Pathogen culturing protocol in selective media. Following the seventh passage step, six (6) colonies from each of the twelve cultures were selected, amplified in liquid media, and the DNA was isolated from each for a total of 72 DNA isolations per strain tested. A frozen archive sample of each clone selected for DNA isolation will also be maintained for potential future analysis.