Synergistic Effects of Salinity and Temperature on the Survival of Two Nonnative Bivalve Molluscs, Perna viridis (Linnaeus 1758) and Mytella charruana (d’Orbigny 1846)

Table 1

(a) Summary of Mytella charruana thermal experiments. (b) Summary of Perna viridis and (c) Mytella charruana temperature-salinity interaction experiments. Acclimation period was the number of days in holding tanks prior to start of experiments. Adjustment period was the number of days to transition from holding tank temperature (20°C) to treatment temperatures. Experiment period was the number of days trial ran including adjustment period. Capital letters correspond to the experimental treatments presented in Figures 2, 3, and 5.

(a) Mytella charruana

Experiments	Collection sites	Latitude & longitude	Collection temperatures (°C)	Mussel size (mm)	Collection salinity (ppt)	Acclimation period (days)	Adjustment period (days)	Experiment period (days)	Mussels per tank/replicate tanks	Temperatures tested (°C)	Salinities tested (ppt)
Adult	Arlington Lions Club	30.378272′ N	13	20–46	10	13	15	33	25/4	6, 9, 11, and 13	NA
A: cold	Jacksonville, FL	81.620828′ W
Adult	Lonnie Wurn	30.375798′ N	28	22–54	8	13	15	33	25/3	20, 31, and 36	NA
B: warm	Jacksonville, FL	81.585165′ W
Adult	Lonnie Wurn	30.375798′ N	27	25–46	10	13	15	33	25/4	20, 23, 28, and 31	NA
C: warm	Jacksonville, FL	81.525165′ W

(b) Perna viridis

Experiments	Collection sites	Latitude & longitude	Collection temperatures (°C)	Mussel size (mm)	Collection salinity (ppt)	Acclimation period (days)	Adjustment period (days)	Experiment period (days)	Mussels per tank/replicate tanks	Temperatures tested (°C)	Salinities tested (ppt)
Juvenile	Huguenot Park	30.405237′ N	10	12–38	36	5	15	28	10/3	6, 9, and 20	5, 20, 36
A: cold	Jacksonville, FL	81.40321′ W
Juvenile	Huguenot Park	30.405237′ N	10	12–38	36	5	15	28	10/3	20, 31, and 35	5, 20, 36
B: warm	Jacksonville, FL	81.40321′ W
Adult	Municipal Marina	29.891973′ N	18	58–114	35	5	15	28	10/3	6, 9, and 20	5, 20, 35
C: cold	St. Augustine, FL	81.310051′ W
Adult	Municipal Marina	29.891973′ N	28	57–113	37	5	15	28	10/3	20, 31, and 35	5, 20, 37
D: warm	St. Augustine, FL	81.310051′ W

(c) Mytella charruana

Experiments	Collection sites	Latitude & longitude	Collection temperatures (°C)	Mussel size (mm)	Collection salinity (ppt)	Acclimation period (days)	Adjustment period (days)	Experiment period (days)	Mussels per tank/replicate tanks	Temperatures tested (°C)	Salinities tested (ppt)
Juvenile	Fire Station 38	30.390077′ N	17	5–12	5	7	15	28	10/3	6, 9, and 20	5, 22.5, 40
E: cold	Jacksonville, FL	81.641576′ W
Juvenile	Fire Station 38	30.390077′ N	29	4–12	9	7	15	28	10/3	20, 31, and 33	5, 22.5, 40
F: warm	Jacksonville, FL	81.641576′ W
Adult	Fire Station 38	30.390077′ N	16	20–38	5	7	15	28	10/3	6, 9, and 20	5, 22.5, 40
G: cold	Jacksonville, FL	81.641576′ W
Adult	Arlington Lions Club	30.378272′ N	28	20–31	5	7	15	28	10/3	20, 31, and 33	5, 22.5, 40
H: warm	Jacksonville, FL	81.620828′ W