Low Activation of CD8⁺ T Cells in response to Viral Peptides in Mexican Patients with Severe Dengue

Table 1

Characteristics of dengue virus peptides used in this study.

Peptide ID	Protein	Sequence	Position	Induced response	Reference
P1	E	FKNPHAKKQDVV	519-530	CD4^+ and CD8^+	Sánchez-Burgos et al., 2010
P2	E	RGARRMAIL	687-695	CD8^+ and antibodies	Sánchez-Burgos et al., 2010
P3	E	DFGSVGGVL	699-707	Mostly antibodies	Sánchez-Burgos et al., 2010
P4	NS3	WITDFKGKTVW	1824-1834	CD4^+	Zeng et al., 1996
P5	NS3	TPEGITPAL	1975-1983	CD4^+ and CD8^+	Livingston et al., 1995
P6	NS3	GTSGSPIVNR	1608-1617	CD8^+	Friberg et al., 2011
P7	NS4a	ASIILEFFL	2199-2207	Low CD4^+ and CD8^+ and antibodies	Sánchez-Burgos et al., 2010
P8	NS4a	LRPASAWTL	2271-2279	Mostly antibodies	Sánchez-Burgos et al., 2010
P9	NS4a	CYSQVNPTTL	2337-2346	CD8^+ and antibodies	Sánchez-Burgos et al., 2010
P10	NS4b	GSYLAGAGL	2469-2477	High CD4^+ and CD8^+	Sánchez-Burgos et al., 2010
P11	NS5	VIPMVTQIAMTDTTP	2826-2840	CD4^+ and CD8^+ and antibodies	Sánchez-Burgos et al., 2010
P12	NS5	YMWLGARFL	2967-1975	Mostly antibodies	Sánchez-Burgos et al., 2010
P13	NS5	SYSGVEGEGL	3003-3012	Low CD4^+ and high CD8^+	Sánchez-Burgos et al., 2010
P14	NS5	YFHRRDLRL	3257-3265	Mid CD4^+ and high CD8^+	Sánchez-Burgos et al., 2010

Most peptide sequences were obtained from Sánchez-Burgos et al. (2010). P4, P5, and P6 were selected from Zeng et al., Livingston et al., and Friberg et al., respectively [38–40]. Position refers to the first and last amino acid in the polyprotein. Peptides that induced IFN-γ production in mouse or human CD4 and CD8 cells were selected for the present study. Some peptides that induced antibody production and low CD4 and CD8 cell activity were selected as controls of activation. CD8 cells were not tested.