Serum sPD1 and sPDL1 as Biomarkers for Evaluating the Immune State of Lung Adenocarcinoma Patients

Table 2

The primer sequences for RT-PCR.

Gene	Sequence (5-3)	Accession
IFN-γ-F	AGTGATGGCTGAACTGTCGC	NM_000619.3
IFN-γ-R	ACTCCTTTTTCGCTTCCCTGT
TNF-α-F	GAGGCCAAGCCCTGGTATG	NM_000594.4
TNF-α-R	CGGGCCGATTGATCTCAGC
T-bet-F	TGAGGCTGAGTTTCGAGCAG	NM_013351.2
T-bet-R	CTGGCCTCGGTAGTAGGACA
IL13-F	CAGAGGATGCTGAGCGGATT	NM_001354993.2
IL13-R	AAACTGGGCCACCTCGATTT
IL5-F	CAGGGAATAGGCACACTGGA	XM_047417148.1
IL5-R	AGTCTTTCCACAGTACCCCCT
IL4-F	CTCGCCTACAAAGCCCAGAG	NM_001354990.2
IL4-R	GTGTCCTTCTCATGGTGGCT
GATA3-F	ACAGAACCGGCCCCTCATTA	XM_047425045.1
GATA3-R	TCCAGAGTGTGGTTGTGGTG
iNOS-F	CGCATGACCTTGGTGTTTGG	NM_000625.4
iNOS-R	CATAGACCTTGGGCTTGCCA
IL12-F	GATAAAACCAGCACAGTGGAGGC	NM_000882.4
IL12-R	GCCAGGCAACTCCCATTAGTT
Arg1-F	GTCTGTGGGAAAAGCAAGCG	NM_000045.4
Arg1-R	CACCAGGCTGATTCTTCCGT
IL10-F	CTGAGAACCAAGACCCAGACA	NM_001382624.1
IL10-R	GATGTCAAACTCACTCATGGCT
TGF-β-F	CTAATGGTGGAAACCCACAACG	XM_011527242.3
TGF-β-R	TATCGCCAGGAATTGTTGCTG
β-Actin-F	TGGCACCCAGCACAATGAA	NM_001101.5
β-Actin-R	CTAAGTCATAGTCCGCCTAGAAGCA