Long-Term In Vitro Passaging Had a Negligible Effect on Extracellular Vesicles Released by Leishmania amazonensis and Induced Protective Immune Response in BALB/c Mice

Figure 7

BALB/c mice were treated with the 3-dose protocol. (a) Lesion size (millimeter (mm)) measurements (). Each point represents the average of the measurements. ANOVA followed by post hoc Tukey’s test; : nonimmunized infected mice compared with IVD-P; : adj compared with mice immunized with LT-P; : adj compared with the group immunized with IVD-P. (b) Parasite load (). Bars denote the average of 9 measurements, and error bars denote the SD. Kruskal-Wallis test followed by Dunn’s test, compared with nonimmunized mice. (c) Production of IgG1 and IgG2a isotype antibody anti-EVs. (d) Measurements of the IgG1 and IgG2a isotype anti-total extract of L. amazonensis in mice immunized and infected with the parasite. Bars represent the average of 9 measurements, and error bars show the SD. Two-way ANOVA followed by post hoc Sidak’s test . Nonimmunized: nonimmunized infected mice; Adj: mice immunized with adjuvant; EVs-LT-P: mice immunized with EVs released by LTP-P; EVs-IVD-P: group of mice immunized with EVs from IVD-P. Data are representative of 2 independent experiments.

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