Long-Term In Vitro Passaging Had a Negligible Effect on Extracellular Vesicles Released by Leishmania amazonensis and Induced Protective Immune Response in BALB/c Mice

Figure 6

BALB/c mice were managed with the two-dose protocol. (a) Footpad size (millimeter (mm)) measurements (). Each point represents the average of the measurements. ANOVA followed by a post hoc Tukey’s test; : adj compared with mice immunized with LT-P; : nonimmunized infected mice compared with the group immunized with LT-P. (b) Parasite load (). Bars denote the average of measurements, and error bars denote the SD. Kruskal-Wallis test followed by Dunn’s test; compared with the nonimmunized group. (c) Production of IgG1 and IgG2a isotype antibody anti-EVs after 2 doses of EV immunizations. (d) Measurement of the IgG1 and IgG2a isotype antitotal extract of L. amazonensis in mice immunized and challenged with the parasite. Bars represent the average of 7 measurements, and error bars show the SD. Two-way ANOVA followed by post hoc Sidak’s test . Nonimmunized: nonimmunized infected mice; Adj: mice immunized with adjuvant; EVs-LT-P: mice immunized with EVs released by LTP-P; EVs-IVD-P: group of mice immunized with EVs from IVD-P. Data are representative of 3 independent experiments.

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