Large-Scale Screening in General Population Children for Celiac Disease with a Multiplex Electrochemiluminescence (ECL) Assay

Figure 1

Illustration of ECL assay. (a) Single autoantibody ECL assay. The autoantibodies in serum bridge the sulfo-tagged antigen to the biotinylated antigen which will be captured by the streptavidin-coated plate. Detection of plate captured sulfo-tagged antigen is accomplished with ECL. (b) Multiplex autoantibody ECL assay. The autoantibodies in serum bridge the sulfo-tagged antigen to the biotinylated antigen which is coupled with a streptavidin-attached specific linker. Each specific linker coupled antibody-antigen complex will be limited in a designated spot area of each well. Detection of plate captured sulfo-tagged antigens is accomplished with ECL.

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