The SIRT3 and SIRT6 Promote Prostate Cancer Progression by Inhibiting Necroptosis-Mediated Innate Immune Response

Figure 4

Necroptosis-induced innate immune cell recruitment controlled by both SIRT3 and SIRT6 is required to suppress prostate cancer progress. (a) Growth curves of xenografted tumors (LNCaP) after induction of control, SIRT3, or SIRT6 shRNA expression with doxycycline in vivo. Relative tumor volumes were calculated by normalizing against the tumor volume at day 1 following doxycycline administration. Body weight was tested daily. (b) Representative experiment of flow cytometric analysis of T cells, macrophages, and neutrophils in doxycycline-induced xenografted tumors (LNCaP). (c) The total number of T cells, macrophages, and neutrophils was showed in the histograms. Results represent of three independent experiments. . (d) The relative expression of CCL8 and CXCL2 is shown in the histograms. GAPDH was used as the internal control. Results represent of three independent experiments. and .

(a)

(b)

(c)

(d)