A Multiple Antigen Peptide Vaccine Containing CD4⁺ T Cell Epitopes Enhances Humoral Immunity against Trichinella spiralis Infection in Mice

Figure 5

Tfh cells, GC B cells, Tfr cells, and Treg cells in the splenocytes and ILN lymphocytes of immunized mice were determined by flow cytometric analysis seven days after the final immunization. MAP-TB vaccination enhanced expansion of Tfh cells and GC B cells, while decreasing the frequencies of Tfr cells and Treg cells compared with the MAP-B or PBS group. For Tfh, Tfr, and Treg analysis, CD3⁺CD4⁺ T cells were gated and the gating strategies are shown in (a). (a) Representative dot plots of Tfh cells gated on CD4⁺ T cells of spleens and ILNs. Percentages in the upper right quadrant indicated the frequencies of CXCR5⁺PD-1⁺CD3⁺CD4⁺ Tfh cells within total CD4⁺ T cells. (b) Representative dot plots of GC B cells gated on B220⁺ B cells of the spleens. Percentages in the upper right quadrant indicated the frequencies of GC B cells within total B220⁺ B cells. (c) Representative dot plots of Tfr cells gated on CD4⁺ T cells of the spleens and ILNs. Percentages in the upper right quadrant indicated the frequencies of CXCR5⁺FoxP3⁺CD3⁺CD4⁺ cells within the CD4⁺ T cells of the spleens and ILNs. (d) Representative dot plots of Treg cells gated on CD4⁺ T cells of the spleens. Percentages in the upper right quadrant indicated the frequencies of CD25⁺FoxP3⁺CD3⁺CD4⁺ cells within the total CD4⁺ T cells of the spleens. The graphs are shown as the (one representative experiment out of three). ; ().

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