Reverse Immunology Approach to Define a New HIV-gp41-Neutralizing Epitope

Figure 7

Neutralizing properties of purified IgG from mice immunized with the Mim_F8-1 phagotope. Dose-response curves of total purified IgG from poor (Mouse no. 2) and good (Mouse no. 4) responder mice tested to neutralize JRCSF (a) and YU-2 (b) HIV-1 strains. Neutralizing activity was performed as in Figure 1. Experiments were run in triplicate and bars indicate SD. (c) Reactivity of the purified IgG at 100 and 300 μg/mL from the good responder mouse, before (black bar) and after depletion of the W614A-3S Abs (grey bar). Neutralizing activity was performed as above using the JR-CSF strain. Experiments were run in duplicate and bars indicate SD.

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