Efficient Acquisition of Fully Human Antibody Genes against Self-Proteins by Sorting Single B Cells Stimulated with Vaccines Based on Nitrated T Helper Cell Epitopes

Figure 4

HER2-NitraTh elicits HER2-specific IgG⁺ B cells in vivo. NCG mice were engrafted with PBMCs on day 0 and DCs on day 7 to build DC-HIS mice. Then, DC-HIS mice were immunized with HER2-NitraTh or HER2-Th on days 7 and 21. (a, b) The sera of vaccinated mice were collected weekly, and the production of HER2-specific human IgM and IgG were evaluated at 400 titer by ELISA. , , and compared with PBS; ^# and ^## compared with HER2-Th. (c, d) Splenocytes were harvested from vaccinated mice on day 28, and the generation of HER2-specific IgM-secreting B cells and IgG-secreting B cells was detected by ELISpot assay. compared with PBS. Data were expressed as ().

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