Mutated p53 Promotes the Symmetric Self-Renewal of Cisplatin-Resistant Lung Cancer Stem-Like Cells and Inhibits the Recruitment of Macrophages

Figure 5

mt P53 promoted Cr-LCSC xenograft progression in nude mice and prevented the accumulation of macrophages at the tumor site. (a) The appearance of subcutaneous tumors in H1299/Cr-mt-p53 LCSC and H1299/Cr-wt-p53 LCSC xenografts. Four out of 6 mice formed subcutaneous tumors after the injection of H1299/Cr-wt-p53 LCSCs, while 6 out of 6 mice formed subcutaneous tumors after the injection of H1299/Cr-mt-p53 LCSCs. (b) Tumor volume growth curves of tumors developed in the xenograft models injected with H1299/Cr-mt-p53 and H1299/Cr-wt-p53 LCSCs. (c) Immunofluorescence staining showing the accumulation of CD68⁺ macrophages in H1299/Cr-wt-p53 LCSC xenografts and the scant accumulation of macrophages in H1299/Cr-mt-p53 LCSC xenografts. . (d) IHC staining showed decreased CCR2 expression levels in H1299/Cr-mt-p53 LCSC xenografts. .

(a)

(b)

(c)

(d)