Mutated p53 Promotes the Symmetric Self-Renewal of Cisplatin-Resistant Lung Cancer Stem-Like Cells and Inhibits the Recruitment of Macrophages

Figure 2

The isolation and continuous culture of Cr-LCSCs obtained from A549/Cr, H460/Cr, and H661/Cr cells. (a) Representative FACS plots demonstrating the isolation of CD44⁺/CD90⁺ LCSCs from A549/Cr, H460/Cr, and H661/Cr cells. (b) Bright field images of spheres generated from clonal density cultures of A549/Cr-LCSCs, H460/Cr-LCSCs, and H661/Cr-LCSCs collected 21 days after the start of the suspension cultures (100x magnification). (c) The number of A549/Cr-LCSC, H460/Cr-LCSC, and H661/Cr-LCSC spheres in 100 μl samples. The data are presented as the for triplicate counts. compared with A549/Cr-LCSCs and ^□ compared with H460/Cr-LCSCs. (d) The sizes (μm) of the A549/Cr-LCSC, H460/Cr-LCSC, and H661/Cr-LCSC spheres. compared with A549/Cr-LCSCs and ^□ compared with H460/Cr-LCSCs. (e) H460/Cr-LCSCs and H661/Cr-LCSCs were labeled with BrdU and identified with an anti-BrdU antibody. .

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