Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout

Figure 3

MSU directly reduced mCD14 expression on PBMCs. (a, b) PBMCs were harvested from healthy volunteers and stimulated with with PBS, MSU (30 μg/mL, 100 μg/mL, and 300 μg/mL), or LPS (0.1 μg/mL, 1 μg/mL, and 10 μg/mL) for 16 h. CD14 expression on PBMCs was detected by flow cytometry (a, c), and the CD68-positive cells were also gated to analyze the CD14 expression by flow cytometry (b, d). Data from three independent experiments are presented as mean. The unpaired Student t-test was used to calculate the significance.

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