Research Article
Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors
Table 1
Panel of antibodies for mass cytometry.
| # | Antigen | Isotope | Clone | Staining | Company |
| 1 | CD1c (BDCA-1) | 166Er | AD5-8E7 | Surface | Miltenyi Biotec | 2 | CD3 | 154Sm | UCHT1 | Surface | Fluidigm | 3 | CD4 | 145Nd | RPA-T4 | Surface | Fluidigm | 4 | CD8a | 146Nd | RPA-T8 | Surface | Fluidigm | 5 | CD11b (Mac-1) | 209Bi | ICRF44 | Surface | Fluidigm | 6 | CD11c | 152Sm | Bu15 | Surface | BioLegend | 7 | CD14 | 151Eu | M5E2 | Surface | Fluidigm | 8 | CD15 | 173Yb | MEM-158 | Surface | Exbio | 9 | CD16 | 148Nd | 3G8 | Surface | Fluidigm | 10 | CD19 | 142Nd | HIB19 | Surface | Fluidigm | 11 | CD25 | 169Tm | 2A3 | Surface | Fluidigm | 12 | CD39 | 160Gd | A1 | Surface | Fluidigm | 13 | CD45 | 89Y or 156Gd | HI30 | Surface | Fluidigm | 14 | CD45RA | 155Gd | HI100 | Surface | Fluidigm | 15 | CD45RO | 164Dy | UCHL1 | Surface | Fluidigm | 16 | CD56 (NCAM) | 176Yb | N901 | Surface | Fluidigm | 17 | CD62L (L-selectin) | 153Eu | DREG-56 | Surface | Fluidigm | 18 | CD68 | 171Yb | Y1/82A | Intracellular | Fluidigm | 19 | CD69 | 144Nd | FN50 | Surface | Fluidigm | 20 | CD127 (IL-7Rα) | 149Sm | A019D5 | Surface | Fluidigm | 21 | CD141 (BDCA-3) | 165Ho | AD5-14H12 | Surface | Miltenyi Biotec | 22 | CD152 (CTLA-4) | 161Dy | 14D3 | Intracellular | Fluidigm | 23 | CD197 (CCR7) | 159Tb | G043H7 | Surface | Fluidigm | 24 | CD275 (ICOS-L) | 163Dy | 2D3/B7-H2 | Surface | BD Biosciences | 25 | CD278 (ICOS) | 143Nd | DX29 | Surface | Fluidigm | 26 | CD279 (PD-1) | 175Lu | EH12.2H7 | Surface | Fluidigm | 27 | CD303 (BDCA-2) | 147Sm | 201A | Surface | Fluidigm | 28 | CD366 (Tim-3) | 150Nd | F38-2E2 | Surface | BioLegend | 29 | Foxp3 | 162Dy | 259D/C7 | Intracellular | Fluidigm | 30 | HLA-DR | 170Er | L243 | Surface | Fluidigm | 31 | Ki-67 | 172Yb | B56 | Intracellular | Fluidigm |
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In-house conjugation with isotope tags applying the Maxpar Antibody Labeling Kit (Fluidigm). |