A Novel Anti-EGFR mAb Ame55 with Lower Toxicity and Better Efficacy than Cetuximab When Combined with Irinotecan

Table 1

Equilibrium dissociation constant (KD) values for affinity, avidity, or binding activity parameters of cetuximab and Ame55 binding to different EGFRs.

Antigen	His-rEGFR	Fc-rEGFR	A431 cells
Method	Biacore 3000		Cell ELISA
Cetuximab (IgG)	1.24 nM	BDL	0.46 nM
Cetuximab (Fab)	ND	1.39 nM	ND
Ame55 (IgG)	468 nM	0.23 nM	1.7 nM
Ame55 (Fab)	ND	931 nM	ND
Nimotuzumab (IgG)	ND	ND	7.6 nM

Notes: ND: not detected; BDL: below the detectable limit (0.1 nM).