Research Article

High-Titre Neutralizing Antibodies to H1N1 Influenza Virus after Mouse Immunization with Yeast Expressed H1 Antigen: A Promising Influenza Vaccine Candidate

Figure 3

Size exclusion chromatography of the H1 antigen on Superdex 200 10/300 GL column. Chromatogram of the IMAC: elution fractions (upper plot) and molecular weight standards (lower plot). Fractions of the H1 protein were liophilised and dissolved in water, followed by injection into a Superdex 200 10/300 GL column preequilibrated with 10 mM Tris pH 7.6 with 200 mM NaCl. Absorbance at 280 nm is shown.