Research Article

High-Titre Neutralizing Antibodies to H1N1 Influenza Virus after Mouse Immunization with Yeast Expressed H1 Antigen: A Promising Influenza Vaccine Candidate

Figure 1

SDS-PAGE analysis of H1 antigen after IMAC purification. Protein was eluted with 250 mM imidazole. Collected fractions were analysed on 4-12% SDS-PAGE following Coomassie staining in reducing (a) and nonreducing (b) conditions. M: molecular weight marker; E: H1 protein eluted from Ni-NTA column by 250 mM imidazole. Fractions with H1 protein were pooled and lyophilized after being run on 4-7% Native PAGE (c).
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(b)
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