Anti-PLA2R1 Antibodies Containing Sera Induce In Vitro Cytotoxicity Mediated by Complement Activation

Figure 3

Predictive factors of anti-PLA2R1-mediated cytotoxicity in immunofluorescence cytotoxicity assay. (a) Relationship between anti-PLA2R1-induced cytotoxicity mediated by complement and epitope spreading profile at baseline (). CysR: immunized against CysR domain alone; CysRC1: immunized against CysR and CTLD1 domains; CysRC1C7: immunized against CysR, CTLD1, and CTLD7domains. (b) Relationship between anti-PLA2R1-induced cytotoxicity mediated by complement and Anti-PLA2R1 IgG4 (RU/ml) titers at baseline (). (c) Relationship between anti-PLA2R1-induced cytotoxicity mediated by complement and Anti-PLA2R1 total IgG titer at baseline (). (d) Correlation between Anti-PLA2R1 total IgG titer and complement-mediated cytotoxicity (). (e) Anti-PLA2R1-induced cytotoxicity mediated by complement according to anti-PLA2R1 IgG subclasses at baseline (). (f) Anti-PLA2R1-induced cytotoxicity mediated by complement regrouped by levels of cytotoxicity according to anti-PLA2R1 IgG subclasses at baseline (). Note that patients with high level of cytotoxicity (≥40%) are predominantly IgG4+IgG1-IgG3.

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