Research Article
Synergistic Effects of Cabozantinib and EGFR-Specific CAR-NK-92 Cells in Renal Cell Carcinoma
Figure 1
Generation and characterization of EGFR-specific CAR-NK-92 cells. (a) Structure diagram of EGFR-specific CAR. EF1α: promoter; SP: signal peptide; scFv: single-chain variable fragment. (b) Transduction efficiency of lentivirus in NK-92 cells. NK-92 cells were transduced with empty lentivirus vector (Ctrl-NK-92) or lentivirus containing the EGFR-specific CAR encoding sequence (CAR-NK-92) and selected with puromycin. The GFP expression was verified by FACS analysis. (c) GFP expression in lentivirus-transduced NK-92 cells in (b) was determined with a fluorescence microscope. The images were taken under ×400 magnification. (d) Western blotting analysis of the CAR expression in NK-92 cells with a monoclonal anti-human CD3ζ antibody. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was also detected as an internal control.
(a) |
(b) |
(c) |
(d) |