LPS Cooperates with Poly-L-Arginine to Promote IL-6 and IL-8 Release via the JNK Signaling Pathway in NCI-H292 Cells

Figure 1

Activation of the JNK signaling pathway by PLA or LPS in NCI-H292 cells. The JNK signaling pathway is activated by 5 μg/mL PLA, 5 μg/mL LPS, or 5 μg/mL PLA + 5 μg/mL LPS in NCI-H292 cells. Western blot analysis showed expression of JNK and the phosphorylated form of JNK (p-JNK) at various time points (0, 15, 30, 45, 60, 90, and 120 min). β-actin was used as an internal protein loading control. ((a) and (b)) The expression level of phosphorylated JNK in NCI-H292 exposed to PLA was increased. ((c) and (d)) The expression level of phosphorylated JNK in NCI-H292 exposed to LPS was increased. ((e) and (f)) The expression level of phosphorylated JNK in NCI-H292 exposed to PLA+LPS was increased prominently. Statistical analyses were compared with the beginning time point: , , and .

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