Regulation of Neutrophil Degranulation and Cytokine Secretion: A Novel Model Approach Based on Linear Fitting

Table 2

Time-dependent (a) secretion of cytokines and (b) granules expressed in ratio between LPS-stimulated and nonstimulated cells ± SEM of at least 3 independent experiments, significantly different from nonstimulated control at the same time point: , , , and .

(a)

Relative secretion	0 h LPS	2 h LPS	4 h LPS	6 h LPS	12 h LPS	24 h LPS
TNFα	1.28 ± 0.28	6.30 ± 1.81	24.82 ± 8.26	51.54 ± 14.79	36.70 ± 11.20	16.48 ± 11.35
IL1α	0.73 ± 0.38	1.43 ± 0.21	3.36 ± 1.92	6.84 ± 4.92	6.67 ± 3.51	14.70 ± 8.51
IL1β	0.69 ± 0.69	1.64 ± 0.2	4.87 ± 2.55	8.45 ± 5.89	7.89 ± 2.3	4.20 ± 3.46
IL6	0.93 ± 0.18	1.30 ± 0.37	34.35 ± 23.38	69.92 ± 50.91	185.53 ± 62.99	23.64 ± 10.51
IL12β	1.02 ± 0.06	0.88 ± 0.04	1.06 ± 0.09	1.16 ± 0.19	6.20 ± 3.57	1.08 ± 0.25
CCL2	0.97 ± 0.13	0.86 ± 0.17	1.11 ± 0.18	1.17 ± 0.36	6.43 ± 1.48	1.69 ± 0.66
CCL3	1.43 ± 1.43	6.72 ± 0.98	24.35 ± 4.51	66.06 ± 17.86	65.98 ± 24.41	11.05 ± 4.62
CCL4	1.06 ± 0.07	2.65 ± 0.76	8.10 ± 3.09	11.69 ± 5.84	69.14 ± 15.76	1.22 ± 0.72
CCL5	0.88 ± 0.05	1.07 ± 0.24	1.04 ± 0.34	0.89 ± 0.22	0.96 ± 0.18	1.05 ± 0.29
IL8	1.02 ± 0.12	4.89 ± 1.47	9.85 ± 2.31	15.84 ± 3.93	31.83 ± 10.15	27.00 ± 8.89

(b)

Relative degranulation	0 h LPS	2 h LPS	4 h LPS	6 h LPS	12 h LPS	24 h LPS
CD63	1.00 ± 0.42	1.09 ± 0.33	0.97 ± 0.10	1.02 ± 0.12	0.88 ± 0.10	0.92 ± 0.12
CD15	1.00 ± 0.07	1.08 ± 0.14	1.26 ± 0.06	1.23 ± 0.07	1.56 ± 0.13	1.36 ± 0.16
CD66b	1.00 ± 0.06	1.14 ± 0.13	1.37 ± 0.12	1.72 ± 0.14	2.41 ± 0.13	1.87 ± 0.20
CD11b	1.00 ± 0.17	1.29 ± 0.14	1.62 ± 0.09	2.11 ± 0.21	2.57 ± 0.29	2.03 ± 0.34
CD13	1.00 ± 0.13	1.08 ± 0.10	1.14 ± 0.13	1.38 ± 0.14	1.94 ± 0.31	1.76 ± 0.32
CD14	1.00 ± 0.30	1.13 ± 0.33	1.31 ± 0.37	1.58 ± 0.44	1.23 ± 0.35	1.00 ± 0.31
CD18	1.00 ± 0.14	1.26 ± 0.26	1.23 ± 0.17	1.61 ± 0.3	2.22 ± 0.33	1.68 ± 0.27
CD45	1.00 ± 0.12	1.19 ± 0.15	1.39 ± 0.11	1.58 ± 0.16	2.18 ± 0.10	1.84 ± 0.22