Research Article
Heparin Interaction with the Primed Polymorphonuclear Leukocyte CD11b Induces Apoptosis and Prevents Cell Activation
Figure 1
PMNL priming. (a) Rates of superoxide release from separated NC PMNLs after 15 min of stimulation by 1 pM, 1 nM, and 1 μM PAF and HD PMNLs activated with 0.32 × 10−7 M PMA. The changes in optical density were monitored at 549 nm continuously up to 10 min in the presence of 0.08 mM cytochrome C. Data are expressed as nmoles/106 cells/10 min; for HD and PAF stimulated (10−9 M and 10−6 M) versus nonstimulated NC PMNLs (no PAF), . (b) Relative expression of surface CD11b on PMNLs measured by flow cytometry using specific PE-labeled antibody, as described in Section 2. Data are expressed as mean fluorescent intensity (MFI); for HD and PAF stimulated (10−9 M and 10−6 M) versus nonstimulated NC PMNLs (no PAF), .
| (a) |
| (b) |