Inhibition of Neutrophil Collagenase/MMP-8 and Gelatinase B/MMP-9 and Protection against Endotoxin Shock

Figure 1

P2 and Inhibitor 2 protect Swiss mice from endotoxin shock after intravenous administration. In all the experiments, 35 mg/kg Regasepin 2 by intravenous injection was included as a positive control peptide. The protective effects of P2 at a dosage of 90 mg/kg (a), 30 mg/kg (b), and 10 mg/kg (c) by intravenous injection were shown. (d) The effect of 35 mg/kg Inhibitor 2 by intravenous injection was evaluated in a similar way as in panel (a) to (c). Kaplan-Meier survival curves were generated. The results of two parallel and independent experiments were pooled ( for each condition). The details of the animal experiments were tabulated in Table 2. All peptides and 0.9% NaCl control solution were administrated intravenously 5 minutes after LPS injection.

(a)

(b)

(c)

(d)