Human CD38^hiCD138⁺ Plasma Cells Can Be Generated In Vitro from CD40-Activated Switched-Memory B Lymphocytes

Table 1

CD31⁺ and CD39⁺ cell frequencies within plasma cells in vitro and in vivo.

	Phenotype of CD38^hi CD138^+/− cells^3
Cell origin^1	Subsets^2		CD39		CD31
	CD138	(%)	MFI	(%)	MFI	(%)
CD70	neg	23 ± 3	515 ± 127	81 ± 6	180 ± 19	29 ± 7
	+	21 ± 4	687 ± 78	91 ± 2	568 ± 108	77 ± 2
CD154	neg	26 ± 3	512 ± 100	81 ± 6	140 ± 23	25 ± 7
	+	18 ± 2	694 ± 69	91 ± 2	526 ± 72	74 ± 3
Blood	neg	0.16 ± 0.02	44 ± 14	34 ± 5	310 ± 44	81 ± 5
	+	0.15 ± 0.03	59 ± 11	34 ± 5	1547 ± 223	93 ± 2
Bone marrow	neg	2.17 ± 0.28	9 ± 0.3	34 ± 2	67 ± 7	43 ± 2
	+	0.41 ± 0.08	52 ± 2.8	54 ± 2	194 ± 12	80 ± 3

B lymphocytes following in vitro differentiation in the presence of CD70 () or CD154 () for 11 and 14 days or mononuclear cells isolated from peripheral blood () and bone marrow samples ().
²The frequency of CD38^hi cells was further analyzed in relation to the expression of CD138.
³CD31 and CD39 frequency and MFI were determined on CD38^hiCD138⁻ (neg) and CD38^hiCD138⁺ (+) as indicated.