Human CD38^hiCD138⁺ Plasma Cells Can Be Generated In Vitro from CD40-Activated Switched-Memory B Lymphocytes

Figure 6

CD31 as an in vivo and in vitro generated plasma cell marker. Analyses were done on cells submitted to 14 days in differentiation conditions. (a) D14 viable cells were analyzed based on their CD31 and CD39 expression. Plots are representative of 10 independent experiments for the CD70 interaction and 13 for the CD154 interaction. (b) Bone marrow and peripheral blood plasma cells (viable CD45⁺). Plots are representative of 10 and 14 independent experiments for bone marrows and for peripheral blood, respectively. (c) Comparison of CD31 and CD39 expression and frequency on generated (CD70 and CD154) and in vivo (PB and BM) plasma cells. CD138⁻ (−) and CD138⁺ (+) plasma cells expression profiles were compared using the Unpaired - or the Mann-Whitney test (indicating a significant difference with values < 0.03). The expression profile of CD138⁻ (−) cells from different origins was compared (indicating significant differences with values < 0.02) as well as CD138⁺ (+) plasma cells (indicating significant differences with values < 0.006) using the Kruskal-Wallis test.

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